CRISPR technology and bioanalytical advances


【讲座题目】CRISPR technology and bioanalytical advances

【时  间】2024年3月29日    上午:10:30-12:00

【地  点】保定校区 国际会议中心3号会议室

【主讲人】X. Chris Le,加拿大皇家科学院院士,首席科学家,Alberta大学杰出教授


X. Chris Le教授自1995年以来,在加拿大阿尔伯塔大学 (University of Alberta) 医学院任教,并在理学院化学系和公共健康学院环境健康专业兼职,于2006年创建分析与环境毒理交叉学科研究室、长期担任主任。 在生命分析化学、环境科学、分子毒理、公共卫生与健康等领域发表文章300余篇。Le教授曾获得加拿大自然科学与工程研究院颁发的青年科学家奖、第一批加拿大首席科学家、加拿大化学会颁发的分析化学成就奖和环境科学成就奖、Alberta大学颁发的优秀导师奖、杰出科研奖、以及大学最高荣誉奖杯等二十多项荣誉和奖励。2010年,Le教授当选为加拿大皇家科学院院士,2011年,当选为Alberta大学杰出教授。


CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and CRISPR-associated (Cas) protein systems have revolutionized genome engineering. CRISPR-Cas systems also advance measurement sciences and diagnostic technology. This presentation will discuss three related topics: a genome-editing nanomachine constructed with a CRISPR system, (2) CRISPR technology incorporated in single-cell RNA sequencing, and (3) integrating CRISPR with isothermal amplification techniques for molecular detection of nucleic acids. These techniques use special features of various Cas proteins, including RNA-guided endonuclease activity, sequence-specific recognition, multiple turnover trans-cleavage activity of Cas12 and Cas13, and unwinding and nicking ability of Cas9. Successful integrations of CRISPR with isothermal amplification techniques, such as loop mediated amplification (LAMP) and recombinase polymerase amplification (RPA), lead to highly sensitive and specific detection of diverse molecular targets.